DEVELOPMENT OF
NEW METHOD of vaccinations and treatment
of infectious diseases with use of the device on the basis of
structure with controlled energy material (CEM)
Authors:
Prof. A.Kozhemyakin, Prof. F.Chernikov and Prof. Yury Tkachenko
Chief of the project is Prof. A.Kozhemjakin
The mechanisms of vaccination, consisting in creation of maximum
specific cellular and humoral immunity against viruses and
bacteria, are well investigated since times of L.Paster. In
simplified form it could be presented as struggle of specific
antibodies and cells of organism immune against alien antigenes.
This method has a number of serious lacks, the main of which
relates to poorly predicted immunity changes with possible
allergization, long period of immunity formation, impossibility for
steady immunity formation at fast mutation of viruses and
bacteria.
Modern idea about mechanisms of interaction between material
objects is related to the dual state of the substance.
First, there is chemical interaction under condition of contact
between objects; second, there is influence of one object on
another with the help of electromagnetic radiation, and in this
case influence can occur on distance without direct contact of the
objects.
The second type of interaction in conditions of low-intensity
radiation is possible exclusively in the case of the coincidence of
object's own frequencies.
Different mechanism for creation of specific immunity is offered -
the material with properties of changed energy structure (СЕМ),
electromagnetic spectrum of which is identical to electromagnetic
spectrum of a virus or bacterium, performs the role of antibody.
When the material with the changed energy structure (СЕМ), which
has electromagnetic radiation spectrum, similar to the alien
infectious agent, is administered into the organism, effect of
vaccination or effect of therapy (through the period of temporary
disease exacerbation) is achieved. This method makes it possible
very quickly to achieve specific immunity from fast mutating
viruses and bacteria, it does not create effect of nonspecific
allergization; the material, administered into the organism, is not
toxic. Thus, there is a real mechanism for high-grade new stage of
struggle against infectious diseases. The most acute opportunities
of the new method are effective fight against fast mutating viruses
and bacteria (SARS, AIDS, etc.) and struggle with especially
dangerous infections, including way of struggle against
bioterrorism. Development of principally new method of vaccination
of living organisms from infectious diseases, and also development
of principally new technology of therapy for infectious diseases
with the maximal antimicrobic and antiviral effects and the minimal
influence on the environmental cells are suggested in the given
project. In the given project it is suggested to use special
semi-conductor structures with controlled energy material (CEM), as
vaccine or treatment.
The resonant frequencies appropriate for DNA, cell membranes, etc.
and its components are found in mm - IR range.
Violation of vital functions of cells of the organism leads to
change of its energy structure in the given range. Each cell of the
organism occupies only appropriated level in its own energy
structure. Accordingly, in the energy spectrum of the organism each
organ occupies only its characteristic area, consisting from the
levels, appropriate for separate cells.
Energy areas of separate human organs are divided by sites of the
spectrum, where radiation is absent. Therefore, with the purpose of
prophylaxis of infectious disease it is suggested that preparation
of "energy analogue" of pathogenic "guest"-microorganism with use
of the device on the basis of structure with controlled energy
material (by method of recording of radiation of
"guest"-microorganism on the material carrier), with its subsequent
incorporation into structure of the protected "host" organism. As
numerous carried out experiments showed, similar procedure leads to
blockade of energy spectrum, characteristic for pathogenic
"guest"-microorganism, and, as a result, to "replacement" of
"guest"-microorganism or another "spectrum guest" structure from
"already occupied" energy level, i.e. to effect of vaccination of
"host"-organism
With the purpose of treatment of infectious disease it is suggested
preparing of similar material and its inclusion into the long
contact with the pacient's "host"-organism. With the purpose of
treatment of infectious disease it is suggested preparing of
similar material and its inclusion into the long contact with the
pacient's "host"-organism.
In this case development of disease passes classical phases of
natural recovery: short-term acute period with synchronization of
pathogenic spectral components (it is necessary to apply profile
pharmacological preparations, which will operate more effectively
in this case), then suppression of vital activity of the "guest"
-microorganism, related with deppression of their energy activity
at all spectral levels, characteristic to them, and with
replacement by radiation of the prepared device.
Duration of treatment depends on the degree of disease development
and features of duplication cycle of pathogenic
"guest"-microorganism.
The control device is created on the basis of molecular fluctuation
light-scattering method for quality control of received recordings
on devices, optimization of recording technology and stability of
work of recording device. Action of this device is based on the
recording-reproduction of spectrum of radiation of
"guest"-microorganism.
The first year of the project realization provides
performance of the following stages of work:
1. Optimization of structure of devices with
controlled energy material. The following things are provided: the
choice of optimum carrier material in the view of recording quality
of electromagnetic (EM) radiation of pathogenic
"guest"-microorganism, technology of the preparation and expenses
for manufacturing and application of the device.
2. Further development of quality monitoring of
availability and quality of radiation recording on the material
carrier is planned.
3. Further improvement of technology of recording
on the material carrier is planned.
4. Selection of components for formation of
"recipe" of recording is planned.
5. Perfomance of wide researches in vitro and on
animals is planned.
Received results will serve as a basis for development and
application of similar devices for prophilaxys and treatment of
infectious diseases and should result in formation of new
pathobiological directions, including medical and veterinary areas.
In the future creation of anticancer vaccines with use of the given
technology is also theoretically possible.
The devices, developed in the project, should be characterized by
simplicity of design, so that there was an opportunity for the
organization of mass and inexpensive manufacture at profile
microelectronics enterprises.
Expected project results of the first
year:
1. The device and technologies of EM radiation
recording of pathogenic cells of "guest"-microorganisms on
different material carriers.
2. Upgrade of the device for evaluation of
quality of recording. The further development of an optical and
electronic device circuit is provided; development of procedure of
measurement and rating results of measurement algorithms (with
development of the software); development of quality rating
recording scales.
3. "Compounding" of preparations for formation of
the electromagnetic recipe for vaccination and treatment.
4. Methods of application of the device with
recording biologically active "recipe".
5. Tests on animals, clinical tests.
The description of the recording
device
The material carrier has 2 steady states, and the first corresponds
to its cold state, and the second is achieved, when the carrier is
given voltage, sufficient for surmounting the energy barrier. The
time sequence of transition of the carrier into excited status (t1)
and return to the initial state after contact with the "guest"
microorganism is given below with the purpose of recording its
radiation with subsequent reproduction.
We used our measuring technique for research of homeopathic
preparations. These researches showed that the applied technique of
measurements made it possible to determine homeopathic preparations
in degrees of dilution up to 10-6000 M.
The opportunity of information transfer of activity of homeopathic
preparations was also achieved. We used also this measuring
technique for estimation of quality of water activation by
different methods. Below you can see the diagrams, which
characterize dynamic condition of water after electrochemical
activation. Distinction of the water, received on anode and on
cathode and at different current value, is shown.
The controlled energy materials, received at transfer of
EM-activity (for example, from "guest" microorganism onto
material), are as easily controlled with the above fluctiation
light-scattering technique as at work with homeopathic
preparations.
Dynamics of morphological changes in the liver at different
variants of opisthorchosis therapy SIBERIAN STATE MEDICAL UNIVERSITY
2002
The purpose of research:
To establish character and dynamics of morphological changes in the
liver at different variants of therapy for opisthorchosis. Research problems:
1. To estimate the character of histological
changes in the liver of hamsters at opisthorchosis before treatment
and after treatment.
2. To make comparative morphological analysis of
necrotic changes in the liver between researched groups.
3. To study character and dynamics of
inflammatory infiltration of portal tracts in the given
groups.
4. To estimate character and dynamics of fibrosis
in the liver in researched groups.
5. To estimate dynamics of histologic changes as
a whole in the liver of hamsters at different variants of
opisthorchosis therapy.
Materials and methods:
The complex comparative morphological analysis of the liver was
conducted in the following groups. The control group consisted of
20 fragments of the liver. Group № 1 is tissue of liver after treatment with
aekarsol (specific drug). Group № 2 is tissue of the liver after treatment
with electromagnetic analogue of opisthorchisis. Group № 3 tissue of the liver after treatment
with electromagnetic (EM) analogue of aekarsol.
Fragments of the liver after sampling were immediately fixed in 12
% neutral formalin. The preparations were made in accordance with a
standard technique, they were covered with paraffin. Cuts 5-6
microns thick were painted with eosin-hematoxylin, picrofuchsin by
Van Gizon.
Necrosis-inflammatory changes and character of fibrosis were
estimated. Necrosis and inflammatory infiltration were referred to
necrosis-inflammatory. The following variants of necrosis were
taken into account: piecemeal necrosis, bridging necrosis and
intra-lobular n ecrosis.
Qualitative (eosinophiles, neutrophils, lymphocytes, monocytes,
fibroblasts) and quantitative calculation of cellular infiltration
of portal tracts, and also density of infiltration in 1 mm2 were
made. The following variants of fibrosis were marked out and taken
into account: portal, septal, (porto-portal, porto-central). Using
function "lasso" in PhotoShop, they calculated the area of portal
tracts, number of cells in infiltration per unit area with
subsequent calculation of specific weight of each cellular form.
For unification of research within the limits of each
micropreparation the estimation was carried out in ten fields of
each third vision field.
Four groups of liver fragments, including control one, "blind"
research was performed. The morphological assessment was on the
device, which included light microscope "Zeiss Jena", digital
camera Epson-200 with the use of program PhotoShop 6.0. The image
of vision field was introduced into computer with the help of
videocamera. The final linear and optical increase after transfer
of via videocamera was х800, 1600.
Results and discussion
On comparing assessment results of necrotic changes in the liver,
the following characteristics were revealed. The therapy with the
use of aekarsol (specific drug), EM analogue of aekarsol and EM
analogue of opisthorchisis was accompanied by positive
dynamics.
So, if in the control group, the number of piecemeal, bridging and
intra-lobular necroses in 10 vision fields was accordingly - 16, 6,
10, at the same time, in the group, treated with aekarsol1 it was
4, 2, 6 (Р?0,05k), and in the group with use of EM analogue of
aekarsol2 - 9, 3, 6 (Р?0,05k), in the group with application of EM
analogue of opisthorchisis 3 - 9, 3, 5 (Р?0,05k)
On comparing parameters of cellular infiltration of portal tracts
among different groups, the following differences were also
revealed.
In control preparations the number of cells per 1 мм2 amounted to
14500 ± 1800, whereas in groups 1, 2, and 3 the number of cells was
6140±810, 8200±980, 8570 ±1050 per unit area accordingly, which was
statistically different.
At comparative assessment of percentage of each cellular form of
inflammatory infiltration the following characteristics were
revealed. If in the control group specific weight of eosinophilic
leucocytes amounted to 48 ±6, in group 1 it was 12 % ±2 (Р?0,05k),
in group 2 - 23 ±%3 (Р?0,05k), and
in group 3 - 13 % ± 2 (Р?0,05k).
Thus in the control group the content of lymphocytes was 20 % ±3,
whereas in group 1 it was 34 % ±4, in group 2 - 40 % ±5, and in
group 3 - 42 % ±6. Distinctions with control group are
reliable.
In comparison with the control group, multiple reduction of
neutrophil specific weight in all groups was observed after
treatment, upon authentic sharp increase of number of
monocytes.
Comparative assessment of fibrosis character revealed that in
groups 1- 3 in comparison with the control group sclerosis evidence
was authentically less.
Thus, if in the control group the number of porto-portal and
porto-central septa in 10 vision fields was 6 to 8, in group 1 it was 2 to 0,
in group 2 it was 3 to 1,
in group 3 it made 3 to 3.
Reliability of distinctions is confirmed statistically (Р?0,05)
During the carried out research we revealed that therapy for
opisthorchosis with use of EM analogue of opisthorchisis and
aekarsol is accompanied by authentic positive dynamics of
morphological picture, which is shown by reduction of inflammatory
infiltration density, obvious decrease of necrotic changes and
extremely weak fibrosis evidence (in comparison with control
group). Application of EM analogues does not cause frequently
observed sclero-gene effect, which is very valuable quality of the
preparations. Thus, both aekarsol therapy and therapy with use of
EM analogue of aekarsol and opisthorchisis, are accompanied by
positive morphological dynamics, significant reduction of not only
necrotic and inflammatory changes, but also of sclerous ones.
VACCINATION FROM TUBERCULOSIS BY CEM
TECHNOLOGY.
EXPERIMENTS ON ANIMALS (MICE)
The ability for vaccinating living organisms from tuberculosis by
electron analog (CEM Technology) of vaccinal culture Micobacterium
bovis (BCG) was checked experimentally with 3 groups of mice.
First group of 20 mice of the inbreed line C57BL/6 was vaccinated
by subcutaneous introduction 2 10 5 alive culture of avirulent
vaccinal culture Micobacterium bovis (BCG). Capsules-carriers with
preliminary recorded signals from culture BCG, were placed on each
mouse in the second group of 20 mice. Third group of 20 mice was
control group. In 5 weeks first and second groups of mice, and also
third control group were infected with intravenous introduction
virulent M.tuberculosis (MBT) culture H37Rv in a doze of 5 10 6.
Before infection, mice of the first and second groups were put to
the tuberculine tests: tuberculine was injected into small pillows
of the left hinder legs and in 24 hours the difference of thickness
left and right (control) small pillows was estimated. It was
revealed that BCG vaccinated mice had the value of hypersensitivity
of slowed down type (HST) equal to 0.17 ± 0.04 mm, and in the
second group it was 0.15 ± 0.08 mm. Both values are significant and
do not differ authentically from each other, which testifies that
the prepared electron carrier is capable "to make" mice immunity
similar to BCG.
Organs of infected mice in the first and the second groups (10 mice
from each group) were taken for histologic researches in 1 month
and 3 months after infection. Within 3 months all mice of the first
two groups remained alive (10 mice in each group), and in the
control group during the first month 12 mice were lost, in 3 months
- others died, which testifies that effect of vaccination was
achieved in the first two groups. Histologic researches have shown
the following: in the control group the process of disease was
characterized by generalization with damage of lungs, liver and
spleen. In the lungs the overall specific pneumonia was found out
as large, merging among themselves, infiltrations with numerous,
breaking apart, neutrophiles, macrophages, lymphocytes, epithelioid
cells, plethora vessels and hypostases. Air pulmonary tissue,
remained between injured sites, made 10-15 % from total volume of
the lung. The mice of the first group, infected with MBT, which
were BCG vaccinated 5 weeks prior to the infection, in 1 month
after infection immune tissue reorganization was detected only as
proliferation of lymphoid and reticular cells in the spleen.
Small proliferation of endotheliacytes was also found out in the
liver. Plethoric capillary network and small diffusive lymphoid and
macrophage infiltration of intersticium was found out in the lungs.
Mice of the same group in 3 months after infection had immunocyte
proliferation already sharply expressed, the spleen increased in 10
and more times. in comparison with the normal one. Follicles were
large, numerous; T- lymphocyte proliferation appeared,
endotheliacytes grew in number, compared to the previous
supervision. Lymphoid focal congestions appeared in the lungs. Air
pulmonary tissue, in relation to the affected tissue, made 80 % of
the total volume.
The mice, infected with MBT, which 5 weeks prior to the infection
and during all the experience were receiving influence of the
capsule-carrier with recorded BCG spectrum, had changes in organs
similar to the mice, vaccinated by the injection.
The tuberculine tests analysis and study of morphological material
have shown, that mice of the first and the second groups had
identical organism reaction to MBT infection. It was completely
different from obzerved organ changes of non-vaccinated animals.
Moreover, mice of the first and the second groups had vivid
differences neither on the degree of immune reorganization, nor on
the morphological picture in the internal organs. It means that
both vaccination methods are identical in their protective
action.
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